Primer

Part:BBa_K4816007:Design

Designed by: Julia Alexander   Group: iGEM23_Bonn-Rheinbach   (2023-09-25)


nosZ primer reverse


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 7
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Specific binding to nosZ gene. Containing of a restriction site for restriction digest and cloning into a plasmid vector after PCR amplification.


Source

Sequence was designed based on the DNA sequence for the nosZ gene of Paracoccus denitrificans found on KEGG https://www.genome.jp/entry/pde:Pden_4219

References